plasmid pet-21d Search Results


cynthia wolberger 42  (Addgene inc)
93
Addgene inc cynthia wolberger 42
Cynthia Wolberger 42, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pet-21d/Ube1%2FPET21d+(Plasmid+%2334965)/ppr0676757-222-5-8
Average 93 stars, based on 1 article reviews
cynthia wolberger 42 - by Bioz Stars, 2026-09
93/100 stars
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biotin ligase  (Addgene inc)
93
Addgene inc biotin ligase
Biotin Ligase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pet-21d/pET21d-myc-BirA+(Plasmid+%23109424)/pmc11014978-197-4-7
Average 93 stars, based on 1 article reviews
biotin ligase - by Bioz Stars, 2026-09
93/100 stars
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unresolved region  (Addgene inc)
93
Addgene inc unresolved region
(A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically <t>unresolved</t> (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.
Unresolved Region, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pet-21d/pET21d-colEdes17%2FImdes17+(Plasmid+%23107125)/pmc09585964-219-4-34
Average 93 stars, based on 1 article reviews
unresolved region - by Bioz Stars, 2026-09
93/100 stars
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bacterial strains pet21d netzer  (Addgene inc)
93
Addgene inc bacterial strains pet21d netzer
(A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically <t>unresolved</t> (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.
Bacterial Strains Pet21d Netzer, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pet-21d/pET21d-colEwt2%2FImwt2+(Plasmid+%23107127)/pm40215973-221-7-13
Average 93 stars, based on 1 article reviews
bacterial strains pet21d netzer - by Bioz Stars, 2026-09
93/100 stars
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pet-21d(+) plasmid carrying recombinant e. coli catalase  (GenScript corporation)
90
GenScript corporation pet-21d(+) plasmid carrying recombinant e. coli catalase
(A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically <t>unresolved</t> (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.
Pet 21d(+) Plasmid Carrying Recombinant E. Coli Catalase, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pet-21d/pet+21d++++plasmid+carrying+recombinant+e++coli+catalase/pmc10594875-122-14-26
Average 90 stars, based on 1 article reviews
pet-21d(+) plasmid carrying recombinant e. coli catalase - by Bioz Stars, 2026-09
90/100 stars
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pdtat.pet21d expression plasmid  (Promega)
90
Promega pdtat.pet21d expression plasmid
(A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically <t>unresolved</t> (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.
Pdtat.Pet21d Expression Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pet-21d/pdtat+pet21d+expression+plasmid/us06846484-200-3-30
Average 90 stars, based on 1 article reviews
pdtat.pet21d expression plasmid - by Bioz Stars, 2026-09
90/100 stars
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pet21d-coledes1/imdes1 (plasmid #107108)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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pet21d-coledes2/imdes2 (plasmid #107109)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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pet21d-coledes16/imdes16 (plasmid #107124)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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pet21d-coledes9/imdes9 (plasmid #107117)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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pet21d-coledes18/imdes18 (plasmid #107126)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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pet21d-coledes8/imdes8 (plasmid #107116)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


(A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically unresolved (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.

Journal: Life Science Alliance

Article Title: An inducible amphipathic α-helix mediates subcellular targeting and membrane binding of RPE65

doi: 10.26508/lsa.202201546

Figure Lengend Snippet: (A) Three-dimensional structure of bovine RPE65 (PDB ID: 3FSN ). The crystallographically unresolved (aa107–125) region of RPE65 protein, modeled using I-TASSER server, is shown in red color. (B) Alignment of RPE65 aa107–125 from several jawed and jawless vertebrate taxa showing sequence conservation throughout evolution. (C) Helical wheel representation of the crystallographically unresolved aa107–125 region of RPE65 protein using HELIQUEST server. (D) Predicted secondary structure, relative accessibility and hydropathy analysis of the aa107–125 region of RPE65 protein using I-TASSER and ENDScript server, respectively. (E) Hydropathic moment plot of combined 35 different RPE65 aa107–125 and paralogous conserved region sequences from vertebrate and non-vertebrate carotenoid cleavage dioxygenase (CCD) paralogs, derived from HeliQuest . Red stars, RPE65 s; magenta circles, vertebrate BCO1s; blue circles, vertebrate BCO2s; black circle, early chordate ( Ciona ) CCD; yellow circles, arthropod ( Trinorchestia , Palaemon , Drosophila , and Galleria ) CCDs; and green circles, nematode ( Caenorhabditis ) -CCDs. Note tight grouping of all 10 RPE65 s distinct from other paralogs. (F) Physicochemical properties of the crystallographically unresolved (aa107–125) of RPE65 protein. Abbreviations: acc, accessibility; hyd, hydrophobicity.

Article Snippet: We cloned the crystallographically unresolved region (107–125 aa, AH 107-125 ) of RPE65 protein into the MBP fusion cloning vector pET His6 MBP TEV LIC (2M-T; a gift from Scott Gradia, plasmid # 29708; Addgene).

Techniques: Sequencing, Derivative Assay